This instructable will explain how to build a fairly basic.
Of course, this device is nothing compared to a commercial spectrophotometer, but it will allow the builder to understand how such a device works to using it for DIYbio.
The spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. Aspectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions, transparent or opaque solids, such as polished glass, or gases.
The basic principle is easy: a beam of light passes through a prism or diffraction grating; out of the resulting spectrum, a range of wavelengths gets selected by sending the light through a slit. The light passes through the sample and hits a detector.
The most common spectrophotometers are used in the UV and visible regions of the spectrum, and some of these instruments also operate into the near-infraredregion as well. Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science.
So, let’s do it!
This device is certainly not to be compared to a commercial system, but if you are a DIYbio fun it will certainly be interesting!
Step 1: Step 1: Materials
For this bit you’re going to need the following.
(I am using modules from the grove kit from seeedstudio)
- 1 x Intel Edison;
- 1 xGrove Base Shield;
- 1 x LED driver board;
- 1 x LED of your choice (I used a color led);
- 1 x Light Sensor;
- 2 x Grove Cable
Step 2: Step 2: Connecting it all together!
Before we get stuck in I am assuming you have your Edison board set up as in Intel guide; if not you can view it here.
Before connecting it all up, I suggest you disconnect the power from the board, it helps prevent any short circuits and other issues.
Firstly, you must connect the Grove Base Shield on Intel Edison Board.
Then, the light sensor needs to connect to port A0 on the expansion board.
Done, it’s all!
For more detail: Intel Edison Spectrophotometer