RWXBioFuge Open Source Centrifugation Machine

Centrifugation is a powerful method for isolation of compounds such as DNA, proteins, oil or membrane vesicles from a complex mixture. The RWXBioFuge was designed to bring this powerful tool into the hands of labtechnicans, scientists, hackers, makers, diybio-ers and those who cannot afford to procure A-label equipment in low-resource settings.

It may also be used as an educational project to teach some fundamental principles of physics, chemistry, biology and electronics hands-on. Apart from an assembly guide, this documentation also includes 7 instructions for demonstration experiments and science classes.

For assembly workshops or any questions, remarks or suggestions please contact [email protected]

This repository contains the code, source files and instructions for operating the RWXBioFuge Open Source microcentrifuge.

  • ArduinoCode: source code for the Arduino Ethernet
  • Rotor: OpenSCAD source code for the rotor, preview at YouMagine
  • Device: Bill of Materials, 3D model, 2D CAD files and Fritzing wiring
  • Docs: assembly and tutorial documents

The V0.1 machine is used in the Waag Society’s Open Wetlab and future improvements will be made during the Open Wetlab open evenings.

WARNING: Fast spinning rotors are dangerous! Make sure you read the safety instructions before use.

LICENSE: All work in this project is licensed under the Creative Commons Attribution-ShareAlike 4.0 International License. In other words: you are free to use everything in this repository as long as you attribute Pieter van Boheemen and share derivative work under the same conditions.

Release notes and features of V1.0

The V1.0 release of the RWXBioFuge contains the following features:

  • Completely Open Source and designed in freely available software, such as Arduino IDE, Sketchup, OpenSCAD, InkScape and Fritzing
  • Constructed out of solely off-the-shelf or digitally fabricated parts
  • High speed centrifuge > 10,000 RPM, depending on your own settings and confidence
  • Configurable speed in percentages of full power
  • Configurable time in convenient steps, including infinite
  • Separate Start and Stop buttons
  • Current RPM, Gforce and remaining time is displayed during operation
  • A button for Short immediate operation
  • Open lid detector
  • Web interface to set, start and stop the machine, including status during operation

For more detail: RWXBioFuge Open Source Centrifugation Machine


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